Their fellow Munster club Bandon RFC claimed the All Ireland title last season and Bruff will hope to emulate their success.
The second semi-final will see Leinster champions Navan and Ulster's Banbridge go to head to head as they bid also for a final spot.
Munster U16 winners Waterpark RFC will also make the short journey to Annacotty as they represent their province taking on Galwegians in their respective semi final clash.
The second semi-final sees Skerries take on Dromore, also on Saturday, at Dundalk RFC and the winners of both fixtures will meet in Naas on 5th April.
All Ireland U17 Semi Final
Sat., 22nd March Waterpark v Galwegians Semi-final, Annacotty, kick-off 12.30pm
Sat., 22nd March Skerries v Dromore, Dundalk, kick-off 1pm
All Ireland U19 Semi Final
Sat. 22nd March Bruff v Sligo Semi-final, Annacotty, kick-off 2.30pm
Sat. 22nd March Navan v Banbridge Semi-final (venue TBC)